Creative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs is offering the most comprehensive services for antibody development projects. With strict regulation and effective execution, we are dedicated to providing the most valuable solutions to complete your projects.
HighlightsCreative Biolabs has established a broad range of platforms for developing novel antibodies or equivalents. These cutting-edge technologies enable our scientists to meet your demands from different aspects and tailor the most appropriate solution that contributes to the success of your projects.
HighlightsWith deep understanding in antibody-related realms and extensive project experience, Creative Biolabs offers a variety of references to help you learn more about our capacities and achievements, including infographic, flyer, case study, peer-reviewed publications, and all kinds of knowledge that can assist your projects. You are also welcome to contact us directly for more specific solutions.
HighlightsGet a real taste of Creative Biolabs, one of the most professional custom service providers in the world. We are committed to providing highly customized comprehensive solutions with the best quality to advance your projects.
HighlightsAt Creative Biolabs, we stand at the forefront of antibody discovery and engineering. With over 20 years of dedicated expertise, we recognize the immense and often untapped potential of chicken Immunoglobulin Y (IgY). Our state-of-the-art De Novo Chicken IgY Sequencing Service provides researchers with the definitive primary amino acid sequence of any IgY antibody, directly from the protein itself, without any need for hybridoma cell lines or genetic information. Leveraging our proprietary mass spectrometry-based platform, we deliver 100% accurate and complete coverage of both the heavy and light of your chicken IgY monoclonal antibody. This critical sequence data is the foundational blueprint for a vast array of applications, from securing intellectual property and developing next-generation diagnostics to humanizing avian antibodies for therapeutic use.
Chicken IgY presents a powerful alternative to mammalian IgG, offering distinct advantages that make it highly valuable for a range of biotechnological applications:
However, harnessing these benefits requires a precise, unambiguous understanding of the antibody's sequence. This is where the challenge—and our solution—begins. De novo sequencing of chicken IgY is a complex undertaking. Unlike traditional IgG, IgY possesses a unique structure, including a larger heavy chain (ν-chain) and different disulfide bond patterns. Furthermore, the high degree of polymorphism in avian immunoglobulin genes can make sequencing via traditional genetic methods unreliable or impossible, especially if the source B-cell line is lost or unavailable. Creative Biolabs overcomes these hurdles with a protein-centric approach, using cutting-edge technology to read the sequence directly from the purified antibody.
Fig. 1 Potential applications of IgY in human health.1
Our De Novo Chicken IgY Sequencing is powered by our proprietary antibody sequencing platform. This integrated system combines advanced protein chemistry, ultra-high-resolution mass spectrometry, and sophisticated bioinformatics to guarantee unparalleled accuracy.
Key Technological Pillars:
Our process is optimized for clarity, speed, and quality, ensuring you are informed at every step.
| Step | Description | Key Deliverable |
| 1. Sample QC & Preparation | Your provided IgY sample (as little as 50 µg required) undergoes rigorous quality control using SDS-PAGE and intact mass analysis to confirm purity and integrity. | Initial QC Report |
| 2. Multi-Enzyme Digestion | The antibody is reduced, alkylated, and digested with a panel of specific proteases to generate comprehensive peptide libraries for both heavy and light chains. | N/A |
| 3. LC-MS/MS Analysis | Each peptide digest is analyzed using high-resolution mass spectrometry to acquire high-quality fragmentation data for millions of peptides. | Raw MS/MS Data Files |
| 4. De Novo Sequence Assembly | Our advanced algorithms assemble the overlapping peptide sequences into the full, contiguous sequence for the variable and constant regions of both chains. | Assembled Full-Length Sequences |
| 5. Data Validation & Reporting | The final sequence is manually verified by our specialists. We confirm full coverage, identify any PTMs, and prepare a comprehensive final report. | Final Comprehensive Report |
Choosing Creative Biolabs gives you a decisive competitive edge. Our service is not just a procedure; it's a comprehensive solution.
| Feature | Creative Biolabs' Platform | Conventional Methods |
| Accuracy | 100% guaranteed accuracy, validated by overlapping peptides and manual review. Distinguishes I/L and other isobaric residues. | Often lower accuracy, susceptible to errors in high-homology regions or incorrect database matching. |
| Coverage | 100% sequence coverage of both variable and constant regions for both heavy (ν) and light (λ) chains. | Often incomplete, with gaps in CDRs or terminal regions. |
| Technology | Ultra-high-resolution MS with multi-protease strategy and advanced bioinformatics. | Older, lower-resolution mass spectrometers; single-enzyme digestion. |
| Sample Requirement | Requires only 50-100 µg of purified antibody protein. No cells needed. | Often requires larger amounts of protein or relies on unstable hybridoma cell lines. |
| Turnaround Time | Industry-leading turnaround times, with projects typically completed. | Can be significantly longer and less predictable. |
| Expertise | 20+ years of dedicated experience in antibody sequencing and engineering. Direct access to our PhD-level scientists. | General service providers with less specialized knowledge of antibody structures. |
The definitive sequence of your chicken IgY antibody is the key to unlocking its full potential across multiple domains:
To further support your research, Creative Biolabs offers a suite of antibody sequencing related services:
Don't let an unknown sequence be the bottleneck in your research. Trust the pioneers in De Novo Antibody Sequencing. Partner with Creative Biolabs to get the fast, accurate, and reliable De Novo Chicken IgY Sequencing data you need to accelerate your project.
Q: What is the required amount and purity of the chicken IgY sample?
A: We recommend providing at least 50 µg of purified IgY at a purity of >90% as determined by SDS-PAGE. Higher amounts and purity levels can further streamline the process.
Q: Can you sequence IgY from a polyclonal mixture?
A: Our standard De Novo Chicken IgY Sequencing service is optimized for monoclonal antibodies. Please contact us to discuss your specific project needs.
Q: How do you differentiate between Leucine (L) and Isoleucine (I)?
A: Our methodology utilizes specific fragmentation techniques and the characteristic fragment ions of Leucine and Isoleucine, allowing for their unambiguous differentiation, which is a critical aspect of 100% accurate de novo antibody sequencing.
Q: What if my IgY is glycosylated or has other modifications?
A: Our platform is designed to identify and characterize a wide range of common post-translational modifications (PTMs), including glycosylation, oxidation, and deamidation. These will be clearly annotated in your final report.
All listed services and products are For Research Use Only. Do Not use in any diagnostic or therapeutic applications.
